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蒲 祥 男 |
职称:副教授 职务:系主任 |
所属系室:应用化学系 |
导师类别:硕士生导师 最高学位:理学博士 |
Tel:13541122813 Email :puxiang@sicau.edu.cn |
< 个人简历 >
毕业学校:中科院/四川大学
招收硕士专业:
学硕:生物资源化学 专硕:生物与医药
招收方向:生物合成化学、绿色催化合成、功能基因组学
已指导硕士学生数:11
主要讲授课程:药物化学,化学生物学,有机化学
学术组织任职:中国化学会会员,中国植物学会会员,Bioorganic Chemistry, Journal of Agricultural and Food Chemistry, Industrial Crops and Products, Plant Physiology and Biochemistry,Phytochemistry Reviews,iScience等期刊审稿人。
四川省岐黄菁英人才,主要从事药用植物中功能性成分的生物合成与绿色制造。主持国家级、省部级科研与教改项目8项,主编英文学术著作1部,副主编、参编规划教材3部,第一或通讯作者在Plant J、Ind Crop Prod等学术刊物上发表SCI收录论文18篇,在大学化学、实验室研究与探索等高等教育期刊发表教研论文5篇,第一发明人授权国家发明专利2项,获软件著作权6件。受邀参加国际与国内学术会议并做学术报告6次。
< 主要论著 >
1. Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production. Applied Microbiology and Biotechnology. 2013,97,9365-9375.
2. Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata. Journal of Industrial Microbiology & Biotechnology. 2015,42,1197-1202.
3. Enhanced production of camptothecin and biological preparation of N 1-acetylkynuramine in Camptotheca acuminata cell suspension cultures. Applied Microbiology and Biotechnology. 2017, 101,4053-4062.
4. Possible clues for camptothecin biosynthesis from the metabolites in camptothecin-producing plants. Fitoterapia. 2019,134,113-128.
5. Biosynthesis-inspired mining and identification of untapped alkaloids in Camptotheca acuminate for enzyme discovery using ultra-high performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry. Journal of Chromatography A, 2020,1620,461036.
6. “双一流”背景下高等农业院校有机化学基础课程教学新模式的构建及实践. 大学化学,2021,36,2002053.
7. Integrative analysis of elicitor-induced camptothecin biosynthesis in Camptotheca acuminata plantlets through a combined omics approach. Frontiers in Plant Science, 2022,13, 851077.
8. Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate. Synthetic and Systems Biotechnology, 2022,7,824-836.
9.“新农科”背景下四川农业大学一流化学专业建设. 大学化学,2022,37,2204075.
10. 有机化学实验,农业农村部“十三五”规划教材. ISBN:9787109302013,副主编.
11. Discovery of unique CYP716C oxidase involved in pentacyclic triterpene biosynthesis from Camptotheca acuminata. Plant Physiology and Biochemistry, 2023,202,107929.
12. Proteomics-guided mining and characterization of epoxidase involved in camptothecin biosynthesis from Camptotheca acuminata. ACS Chemical Biology, 2023,18,1772-1785.
13. Discovery of a novel flavonol O-methyltransferase possessing sequential 4′- and 7-O-methyltransferase activity from Camptotheca acuminata Decne. International Journal of Biological Macromolecules. 2024,266,131381.
14. Hydroxylase-oriented mining and functional characterization of camptothecin 10-hydroxylase from Camptotheca acuminata Decne. Industrial Crops & Products, 2024,222,119469.
15. Multiomics-guided mining and characterization of epoxide hydrolase involved in camptothecin biosynthesis from Camptotheca acuminata. Bioorganic Chemistry, 2024,153,107980.
16. Discovery and enzymatic engineering of two noncanonical flavonoid C3-hydroxylases from Camptotheca acuminata Decne. The Plant Journal, 2025,124, e70536.
17. Functional characterization of loganic acid O-methyltransferase and secologanin synthase from Uncaria rhynchophylla and two-step biosynthesis of secologanin in tobacco. International Journal of Biological Macromolecules, 2025, 333, 148806.
18. 喜树基因库CYP450酶元件发掘、功能表征与酶活改良创新实验设计. 实验室研究与探索,2025, 44,51-59.
< 科研项目及成果获奖(含专利、新品种) >
1.基于转录组的喜树碱合成关键基因CaSTR的挖掘与功能分析,四川省教育厅重点项目,2017-2019,主持
2.喜树碱生物合成关键酶基因CaSTR的挖掘与功能研究,国家自然科学基金项目,2018-2020,主持
3.“双一流”背景下高等农业院校数理化基础课程教学新模式的构建及实践,四川省教育厅教改项目,2018-2020,主持
4. 高等农业院校数理化基础课程“3+3”教学模式的构建与实践,2020年校级教学成果奖二等奖
5.喜树中具有环氧化和羟化氧化功能的CYP450基因的挖掘与功能研究,中央引导地方科技发展项目,2021-2022,主持
6. 羟基喜树碱生物合成关键基因CaCYP81的挖掘与功能研究,四川省自然科学青年科学基金项目,2022-2023,主持
7.钩藤中钩藤碱螺环合成途径双键还原酶元件的发掘与功能解析, 中央本级重大增减支项目, 2024-2026, 主持
8. 钩藤碱螺环合成限速步骤UrOMT酶元件发掘与关键前体缝籽木嗪甲醚的绿色合成,四川省自然科学基金面上项目,2025-2026,主持
9. 喜树基因库中CYP450 酶元件发掘与表征,四川省高等学校创新性实验项目,2024-2026,主持
10. 学创一体、虚实互补、科教融汇——新农科背景下农林高校化学类一流专业建设改革与实践,2025年校级教学成果奖二等奖